Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha.

Gao J, Gao N, Zhai X, Zhou YJ
iScience. 2021 Feb 9;24(3):102168. doi: 10.1016/j.isci.2021.102168. eCollection 2021 Mar 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
160127	pHpgRNA13	To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1
160128	pHpgRNA50	To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1