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Dual DNA and protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia.

Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP
Nat Methods. 2021 Mar;18(3):293-302. doi: 10.1038/s41592-021-01077-8. Epub 2021 Mar 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
160081pTXB1-Tn5-FBacterial expression of C-terminally FLAG-tagged Tn5 transposase
160083pTXB1-APEX2-FBacterial expression of C-terminally FLAG-tagged APEX2 peroxidase
160084pTXB1-TP1Bacterial expression of Tn5-APEX2 fusion protein, no linker
160085pTXB1-TP2Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP
160086pTXB1-TP3Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA
160087pTXB1-TP4Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4
160088pTXB1-TP5Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

Antibodies from Article