lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements.
Gordon MG, Inoue F, Martin B, Schubach M, Agarwal V, Whalen S, Feng S, Zhao J, Ashuach T, Ziffra R, Kreimer A, Georgakopoulous-Soares I, Yosef N, Ye CJ, Pollard KS, Shendure J, Kircher M, Ahituv N
Nat Protoc. 2020 Aug;15(8):2387-2412. doi: 10.1038/s41596-020-0333-5. Epub 2020 Jul 8.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
137724 | pLS-SV40-mP-EGFP | Lentivirus based EGFP expression vector |
137725 | pLS-SceI | Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene. |