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lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements.

Gordon MG, Inoue F, Martin B, Schubach M, Agarwal V, Whalen S, Feng S, Zhao J, Ashuach T, Ziffra R, Kreimer A, Georgakopoulous-Soares I, Yosef N, Ye CJ, Pollard KS, Shendure J, Kircher M, Ahituv N
Nat Protoc. 2020 Aug;15(8):2387-2412. doi: 10.1038/s41596-020-0333-5. Epub 2020 Jul 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
137724pLS-SV40-mP-EGFPLentivirus based EGFP expression vector
137725pLS-SceILentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

Antibodies from Article