Tollip coordinates Parkin-dependent trafficking of mitochondrial-derived vesicles.

Ryan TA, Phillips EO, Collier CL, Jb Robinson A, Routledge D, Wood RE, Assar EA, Tumbarello DA
EMBO J. 2020 Jun 2;39(11):e102539. doi: 10.15252/embj.2019102539. Epub 2020 Apr 20. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
178050	GFP-Tollip	GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.
178051	GFP-Tollip M240A,F241A	GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.
178054	pIRESneo2 mycBioID-Tollip	myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
178055	pIRESneo2 mycBioID-Tollip M240A,F241A	myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
196546	pLentiCRISPRv1 sgTollip	Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.