A Universal Surrogate Reporter for Efficient Enrichment of CRISPR/Cas9-Mediated Homology-Directed Repair in Mammalian Cells.
Yan N, Sun Y, Fang Y, Deng J, Mu L, Xu K, Mymryk JS, Zhang Z
Mol Ther Nucleic Acids. 2020 Mar 6;19:775-789. doi: 10.1016/j.omtn.2019.12.021. Epub 2019 Dec 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208829 | pHDR-USR-SpCas9 | HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR. |
| 208830 | pHDR-USR-eGFP | HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR. |