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Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha.

Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C, Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J
Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 10.1038/s41564-019-0592-5. Epub 2019 Oct 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132928pSIV-D3psi/delta env/delta Vif/delta VprProduction of SIV-VLPs containing Vpx protein
132932pAPM-D4-miR30-TRIM5Lentivector for TRIM5 knockdown and puromycin resistance
132933pABM-D4-miR30-L1221Lentivector for luciferase (control) knockdown and blasticidin resistance
132934pABM-D4-miR30-CypALentivector for CypA knockdown and blasticidin resistance
132935pLXIN GFPMLV-based transfer vector expressing GFP and neomycin resistance protein
132936pPU-Empty-miR30-TRIM5All-in-one control rescue-TRIM5 shRNA knockdown vector
132937pPU-huTRIMmiR30-L1221All-in-one huTRIM5 rescue-control shRNA knockdown
132938pPU-huTRIMmiR30-TRIM5All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown
132939pUC57mini ZM249M P90A _env eGFPpUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence
132940pPU-Empty-miR30-L1221All-in-one control rescue-control shRNA knockdown vector
132941pCIG3 NMLV packaging plasmid for N-tropic MLV gag-pol expression
132942pCIG3 BMLV packaging plasmid for B-tropic MLV gag-pol expression
139285pcDNA-c/o NL4-3 Env delta CTHIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

Antibodies from Article