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Bioassay for Determining the Concentrations of Caffeine and Individual Methylxanthines in Complex Samples

Gutierrez AE, Shah P, Rex AE, Nguyen TC, Kenkare SP, Barrick JE, Mishler DM
Appl Environ Microbiol. 2019 Nov 14;85(23). pii: AEM.01965-19. doi: 10.1128/AEM.01965-19. Print 2019 Dec 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113646pDCAF-ADemethylates methylxanthines.
113647pDCAF-BDemethylates methylxanthines.
113648pDCAF-CDemethylates methylxanthines.
113649pDCAF-ABDemethylates methylxanthines.
113650pDCAF-ACDemethylates methylxanthines.
113651pDCAF-BCDemethylates methylxanthines.
113652pDCAF-ABCDemethylates methylxanthines.
113653delta guaBBase strain that the pDCAF plasmids (Addgene #113646-113652) must be in.

Antibodies from Article