Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.
Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI
Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104593 | pCA528 CHMP1A 140-196 | Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1 |
| 104594 | pCA528 CHMP2A 152-222 | Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3 |
| 104595 | pCA528 CHMP2B 141-213 | Expresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4 |
| 104596 | pCA528 CHMP4B 156-224 | Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7 |
| 104597 | pCA528 CHMP6 145-201 | Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11 |
| 104598 | pCA528 CHMP7 366-453 | Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12 |
| 104599 | pCA528 CHMP3 159-222 | Expresses C-terminal 159-222 residues of CHMP3 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-5 |
| 108255 | pCA528 CHMP1B 143-199 | Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2 |
| 108256 | pCA528 CHMP4A 153-222 | Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6 |
| 108257 | pCA528 CHMP4C 156-233 | Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9 |
| 108259 | pCA528 CHMP5 159-222 | Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10 |
| 160050 | pCA528 AMSH 1-178 | Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22. |
| 160051 | pCAG-OSF-KATNA1 | Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18. |
| 160052 | pCAG-OSF-KATNA1 R14A | Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19. |
| 160053 | pCAG-OSF-KATNA1 V55D | Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17. |
| 160054 | pCAG-Myc-KATNB1 | Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16. |
| 180609 | pCA528 AMSHLP 16-218 | Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83 |
| 180610 | pGEX-THTAP-CALPAIN-7 1-165 | Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129 |
| 180611 | pCA528 CALPAIN-7 residues 1-165 | Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25 |
| 180612 | pCA528 CALPAIN-7 residues 1-165, V18D | Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26. |
| 180613 | pCA528 CALPAIN-7 residues 1-165, F98D | Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27. |
| 180614 | pCA528 KATNA1 MIT residues 1-79 | Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20. |
| 180615 | pCA528 KATNA1 MIT residues 1-79, V55D | Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23. |
| 180618 | pCA528 KATNAL1 MIT residues 1-87 | Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54. |
| 180619 | pCA528 KATNAL2 residues 1-98 | Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28. |
| 180621 | pGEX-THTAP NRBF2 MIT residues 1-111 | Bacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88. |
| 180622 | pCA528 RPS6KC1 MIT, residues 235-316 | Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87. |
| 180623 | pCA528 RPS6KL1 MIT, residues 20-140 | Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32. |
| 180624 | pGEX-THTAP-SNX15, residues 263-342 | Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130. |
| 180625 | pCA528 SNX15 MIT residues 263-342 | Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14 |
| 180626 | pGEX-THTAP-SPARTIN, residues 1-101 | Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57. |
| 180627 | pCA528 SPASTIN , residues 112-196 | Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111. |
| 180628 | pCA528 SPASTIN , residues 108-200 | Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10. |
| 180629 | pCA528 SPASTIN , F124D, residues 108-200 | Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12. |
| 180634 | pCA528 SPASTIN , L177D, residues 108-200 | Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13. |
| 180636 | pCA528 USP8, residues 1-147 | Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86. |
| 180637 | pCA528 USP54, residues 731-878 | Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85 |
| 180638 | pET16B VPS4A, residues 1-84 | Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43 |
| 180639 | pGEX2T VPS4B, residues 1-86 | Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155. |
| 180640 | pGEX-THTAP-VPS9D1, residues 1-97 | Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89. |
| 180641 | pCA528 CHMP4B, residues 156-224, no Cys | Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140 |
| 180642 | pCA528 CHMP4C, residues 156-233, no Cys | Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14. |
| 180643 | pCA528 IST1, residues 315-360, no Cys | Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99. |
| 180644 | pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050 | Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37. |
| 180645 | pCAG-CHMP2A-myc, L216D/L219D | Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13. |
| 180646 | pLVX-mCherry | Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61. |
| 180647 | pLVX-mCherry-SPASTIN | Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43. |
| 180648 | pLVX-mCherry-SPASTIN, F124D | Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44. |
| 180649 | pLVX-mCherry-SPASTIN, L177D | Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45. |
| 180650 | pLVX-mCherry-KATNA1 | Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47. |
| 180651 | pLVX-mCherry-KATNA1, V55D | Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48. |
| 180652 | pLVX-CALPAIN-7-mCherry | Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50. |
| 180653 | pLVX-CALPAIN-7-mCherry, F98D | Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53. |
| 191234 | pCA528 IST1, residues 316-366, C-Cys | Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling |