Synthetic mammalian signaling circuits for robust cell population control.
Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB
Cell. 2022 Mar 17;185(6):967-979.e12. doi: 10.1016/j.cell.2022.01.026. Epub 2022 Mar 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160042 | vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR | For the auxin receiver that report on auxin. |
| 160043 | vYM140_PB-pEFa1-iaaM(P.s)-Hygro | Expressing iaaM for converting L-Trp to IAM |
| 160044 | vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro) | For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA) |
| 160045 | vYM112_PB-pEF1a-optiPIN2-HA(Hygro) | For expressing the active auxin pump PIN2 |
| 160046 | vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR | For expressing the full paradoxical circuit |