Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products.
Rajkumar AS, Morrissey JP
Microb Cell Fact. 2020 Nov 11;19(1):207. doi: 10.1186/s12934-020-01461-7.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160004 | pMTU-DO-URA | Low-copy expression vector for K. marxianus, uracil selection |
| 160005 | pMTU-DO-HIS | Low-copy expression vector for K. marxianus, histidine selection |
| 160006 | pMTU-DO-LEU | Low-copy expression vector for K. marxianus, leucine selection |
| 160007 | pMTU-DO-G418 | Low-copy expression vector for K. marxianus, G418 selection |
| 160008 | pMTU-DO-HPH | Low-copy expression vector for K. marxianus, hygromycin selection |
| 160009 | pI1-MTU-DO-URA | Integrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection. |
| 160012 | pI1-MTU-DO-G418 | Integrative vector for K. marxianus, targeting the LAC4 locus. G418 selection. |
| 160013 | pI1-MTU-DO-LEU | Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection. |
| 160014 | pI1-MTU-DO-HPH | Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection. |
| 160015 | pI2-MTU-DO-URA | Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection. |
| 160016 | pI2-MTU-DO-HIS | Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection. |
| 160017 | pI2-MTU-DO-LEU | Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection. |
| 160018 | pI2-MTU-DO-G418 | Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection. |
| 160019 | pI2-MTU-DO-HPH | Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection. |
| 160020 | pI3-MTU-DO-URA | Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection. |
| 160021 | pI3-MTU-DO-HIS | Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection. |
| 160023 | pI3-MTU-DO-G418 | Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection. |
| 160025 | pI4-MTU-DO-URA | Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection. |
| 160026 | pI4-MTU-DO-HIS | Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection. |
| 160029 | pI4-MTU-DO-LEU | Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection. |
| 160030 | pI6-MTU-DO-URA | Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background. |
| 160142 | pI1-MTU-DO-HIS | Integrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection. |
| 160176 | pI3-MTU-DO-LEU | Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection. |
| 160207 | pI3-MTU-DO-HPH | Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection. |
| 160215 | pI4-MTU-DO-G418 | Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection. |
| 160216 | pI4-MTU-DO-HPH | Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection |
| 160339 | pMTU-DO-URA-C4 | High-copy expression vector for K. marxianus, uracil selection |
| 160340 | pMTU-DO-HIS-C4 | High-copy expression vector for K. marxianus, histidine selection |
| 160341 | pMTU-DO-LEU-C4 | High-copy expression vector for K. marxianus, leucine selection |
| 160342 | pMTU-DO-G418-C4 | High-copy expression vector for K. marxianus, G418 selection |
| 160343 | pMTU-DO-HPH-C4 | High-copy expression vector for K. marxianus, hygromycin selection |