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Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold.

Carrasco-Lopez C, Zhao EM, Gil AA, Alam N, Toettcher JE, Avalos JL
Nat Commun. 2020 Aug 13;11(1):4045. doi: 10.1038/s41467-020-17837-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
159935pCri_L706-antiSH2-OptoMBOpto-MB bacterial expression
159936pCri_L830-antiSH2-OptoMB-V416LOpto-MB bacterial expression
159937pCri_L889-antiSH2-OptoMB-TripleOpto-MB bacterial expression
159938pHR_L706-antiSH2-OptoMB-irFPOpto-Monobody mammalian expression
159939pHR_SH2-mCherry-CAAXSH2 membrane localization

Antibodies from Article