RNA timestamps identify the age of single molecules in RNA sequencing.
Rodriques SG, Chen LM, Liu S, Zhong ED, Scherrer JR, Boyden ES, Chen F
Nat Biotechnol. 2020 Oct 19. pii: 10.1038/s41587-020-0704-z. doi: 10.1038/s41587-020-0704-z.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159922 | pAAV-Ef1a-MCP-huADARE488QT490A | Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5 |
| 159923 | pcDNA3.1-GAVPO | GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133 |
| 159924 | pTRE3G-iRFP-B1-timestamp_A | Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1. |
| 159925 | pLenti-5xUASG-iRFP-B3-timestamp-A | timestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3. |
| 159926 | pTRE3G-c-fos-iRFP-B3-repRNA-A | Timestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187. |