Efficient generation of mouse models with the prime editing system.
Liu Y, Li X, He S, Huang S, Li C, Chen Y, Liu Z, Huang X, Wang X
Cell Discov. 2020 Apr 28;6:27. doi: 10.1038/s41421-020-0165-z. eCollection 2020.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159780 | pGL3-U6-sgRNA_SRD5A1-mcherry | S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene |
| 159781 | pGL3-U6-pegRNA_SRD5A3-EGFP | S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing |
| 159782 | pGL3-U6-sgRNA_SRD5A3-mcherry | S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A3 gene |
| 159784 | pGL3-U6-sgRNA_EMX1-mcherry | S. pyogenes sgRNA collocated with pegRNA targeting human EMX1 gene |
| 159785 | pGL3-U6-pegRNA_FANCF-EGFP | S. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editing |
| 159786 | pGL3-U6-sgRNA_FANCF-mcherry | S. pyogenes sgRNA collocated with pegRNA targeting human FANCF gene |
| 159787 | pGL3-U6-pegRNA_KCNA1-EGFP | S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing |
| 159788 | pGL3-U6-sgRNA_KCNA1-mcherry | S. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 gene |
| 159790 | pGL3-U6-sgRNA_VEGFA-mcherry | S. pyogenes sgRNA collocated with pegRNA targeting human VEGFA gene |
| 159791 | pGL3-U6-pegRNA_HOXD13_CtoG-EGFP | S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing |
| 159792 | pGL3-U6-pegRNA_HOXD13_CtoT-EGFP | S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing |
| 159793 | pGL3-U6-sgRNA_HOXD13-mcherry | S. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 gene |