Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane.
Lakshman B, Messing S, Schmid EM, Clogston JD, Gillette WK, Esposito D, Kessing B, Fletcher DA, Nissley DV, McCormick F, Stephen AG, Jean-Francois FL
J Biol Chem. 2019 Feb 8;294(6):2193-2207. doi: 10.1074/jbc.RA118.005669. Epub 2018 Dec 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159696 | R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131) | E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131) |
| 159697 | R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188) | E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188) |
| 159698 | R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188) | E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188) |
| 159699 | R725-X11-566: His6-MBP-tev-Hs.LGALS3 | E. coli protein expression of His6-MBP-tev-Hs.LGALS3 |
| 159700 | R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3 | E. coli protein expression |