muDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single Cells.
Altemose N, Maslan A, Rios-Martinez C, Lai A, White JA, Streets A
Cell Syst. 2020 Oct 21;11(4):354-366.e9. doi: 10.1016/j.cels.2020.08.015. Epub 2020 Sep 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159599 | DD-DamV133A-LMNB1-IRES2-mCherry | Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam. |
| 159600 | DD-DamV133A-IRES2-mCherry | Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam. |
| 159601 | DD-DamWT-LMNB1-IRES2-mCherry | Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the wild-type allele of Dam. |
| 159602 | DD-DamWT-IRES2-mCherry | Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the wild-type allele of Dam. |
| 159604 | DD-DamV133A-tdTomato-LMNB1 | Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels. |
| 159607 | m6A-Tracer-NES | Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions. |