Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions.
Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG
Sci Rep. 2015 Nov 2;5:15916. doi: 10.1038/srep15916.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159539 | R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) |
| 159540 | R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C |
| 159541 | R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D |
| 159542 | R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V |
| 159543 | R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D |
| 159544 | R989-X12-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L |
| 159545 | R989-X13-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R |
| 159546 | R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) |
| 159547 | R989-X27-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C |
| 159548 | R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D |
| 159549 | R989-X29-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V |
| 159550 | R989-X30-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D |
| 159551 | R989-X31-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L |
| 159552 | R989-X32-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R | E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R |
| 159553 | R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) | Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) |
| 159554 | R989-X27-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C | Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C |
| 159555 | R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D | Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D |
| 159556 | R989-X29-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V | Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V |
| 159557 | R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D | Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D |
| 159558 | R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L | Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L |
| 159559 | R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R | Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R |
| 159560 | R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) |
| 159561 | R701-X53-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C |
| 159562 | R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D |
| 159563 | R701-X55-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V |
| 159564 | R701-X56-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D |
| 159565 | R701-X57-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L |
| 159566 | R701-X58-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R |
| 159567 | R701-X08-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) |
| 159568 | R701-X20-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C |
| 159569 | R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D |
| 159570 | R701-X19-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V |
| 159571 | R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D |
| 159572 | R701-X23-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L |
| 159573 | R701-X24-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R | E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R |
| 159574 | pDest-636 | Gateway Destination vector for protein expression in baculovirus |