Adaptive laboratory evolution of methylotrophic Escherichia coli enables synthesis of all amino acids from methanol-derived carbon.
Har JRG, Agee A, Bennett RK, Papoutsakis ET, Antoniewicz MR
Appl Microbiol Biotechnol. 2021 Jan;105(2):869-876. doi: 10.1007/s00253-020-11058-0. Epub 2021 Jan 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159446 | T-B18 | E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon |