Precise genome engineering in Drosophila using prime editing.

Bosch JA, Birchak G, Perrimon N
Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 10.1073/pnas.2021996118. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
149545	pCFD3-NS	Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
149546	pCFD5-NS	Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
149548	pEntr_PE2	Gateway entry clone with PE2 enzyme (with stop codon)
149549	pNos-PE2-attB	Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.
149550	pUAS-PE2-attB	Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.
149552	pAct-PE2-HygroR	Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.
149610	pAct-GW-HygroR	Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.