Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive beta Cell Destruction.
Ma H, Jeppesen JF, Jaenisch R
Cell Rep Med. 2020 Sep 22;1(6):100097. doi: 10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159280 | AAVS1-PDL1 | Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant. |
| 159281 | CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector | A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) |
| 159283 | CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA | A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS). |
| 159348 | INS-2A-luciferase-2A-tdT | Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant. |
| 159349 | INS-2A-luciferase-2A-CD19-GFP | Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant. |