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Genetically Encoded Fluorescent Biosensor for Rapid Detection of Protein Expression.

Eason MG, Pandelieva AT, Mayer MM, Khan ST, Garcia HG, Chica RA
ACS Synth Biol. 2020 Nov 20;9(11):2955-2963. doi: 10.1021/acssynbio.0c00407. Epub 2020 Oct 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
159373pET11a-STEPtagExpresses STEPtag in E. coli
159374pET11a-STEPtag-L10-TAXExpresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.
159375pET11a-TAX-L10-STEPtagExpresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.
159376pET-gSTEP1Expresses gSTEP1 in E. coli

Antibodies from Article