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Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex.

Chen J, Malone B, Llewellyn E, Grasso M, Shelton PMM, Olinares PDB, Maruthi K, Eng ET, Vatandaslar H, Chait B, Kapoor T, Darst SA, Campbell EA
bioRxiv. 2020 Jul 13. doi: 10.1101/2020.07.08.194084. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
159092pCDFDuet-nsp7-nsp8Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8
159107pRSFDuet-sumo-NSP12 (SARS-CoV-2)The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
159390pET28a 6xHis PreScission SARS-CoV-2 nsp13Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

Antibodies from Article