Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-in.
Chen YJ, Cheng YY, Wang W, Tian XJ, Lefever DE, Taft DA, Zhang J, Xing J
Biol Methods Protoc. 2020 Mar 20;5(1):bpaa006. doi: 10.1093/biomethods/bpaa006. eCollection 2020.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159434 | pcDNA3-P1-LoxP-Neo-N-miRFP670-C | CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker |
| 159436 | pcDNA3-P1-N-EGFP-C | CRISPR knock-in donor construct with fluorescent reporter |
| 159437 | pcDNA3-P1-N-mCherry-C | CRISPR knock-in donor construct with fluorescent reporter |
| 159438 | pcDNA3-P1-N-miRFP670-C | CRISPR knock-in donor construct with fluorescent reporter |