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Membrane voltage dysregulation driven by metabolic dysfunction underlies bactericidal activity of aminoglycosides.

Bruni GN, Kralj JM
eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158979pKL09-GCaMP6f-mScarlet bb118Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.
158980pKL10-mRuby-iATPSnFR1.0 bb118Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.
158981pKL12-GCaMP6f-pHuji bb118Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.
158982pKL13-MgtC bb118Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.
158983pKL11-GCaMP6f bb100Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Antibodies from Article