Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish.

Hans S, Zoller D, Hammer J, Stucke J, Spiess S, Kesavan G, Kroehne V, Eguiguren JS, Ezhkova D, Petzold A, Dahl A, Brand M
Nat Commun. 2021 Feb 18;12(1):1125. doi: 10.1038/s41467-021-21427-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
158961	pBS U6a gRNA	gRNA backbone driven by zebrafish U6a promoter
158962	pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP	Cre-dependet Cas9-GFP expression