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Soma-Targeted Imaging of Neural Circuits by Ribosome Tethering.

Chen Y, Jang H, Spratt PWE, Kosar S, Taylor DE, Essner RA, Bai L, Leib DE, Kuo TW, Lin YC, Patel M, Subkhangulova A, Kato S, Feinberg EH, Bender KJ, Knight ZA, Garrison JL
Neuron. 2020 Jun 5. pii: S0896-6273(20)30351-2. doi: 10.1016/j.neuron.2020.05.005. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158777pyc126mribosome-tagged GCaMP for soma localization
158778pyc128msoluble GCaMP expression
158779pyc111mribosome-tagged, Cre-dependent GCaMP for soma localization
158780pyc111fribosome-tagged, Cre-dependent GCaMP for soma localization
158781pyc111sribosome-tagged, Cre-dependent GCaMP for soma localization
158785pHJ4Pan-neuronal nuclear-localized GCaMP expression in C. elegans
158786pAS16Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)
158787pAS18Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans
158788pAS19Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Antibodies from Article