Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
eLife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158763 | pEF NS34a-msGFP-A'Q-mito | Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease. |
| 158764 | pEF NS34a-msGFP-'Q-mito | Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease. |
| 158765 | pEF NS34a-msGFP-'S-mito | Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease. |
| 158766 | pF(UG) hSyn NS34a-msGFP-A'Q-mito | Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease. |
| 158767 | pF(UG) hSyn NS34a-msGFP-'Q-mito | Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease. |
| 158768 | pF(UG) hSyn NS34a-msGFP-'S-mito | Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease. |
| 158769 | pF(UG) CamKII sf iGluSnFR | iGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane. |
| 158770 | pF(UG) hSyn SYP-SELF | Expression of synaptophysin 1 variant with self-cleaving c-terminal tail. |
| 158771 | pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt) | Expression of synaptotagmin 1 variant with self-cleaving C2 domain. |