Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays.
Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
Protein Expr Purif. 2020 Jun 4;174:105686. doi: 10.1016/j.pep.2020.105686.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166010 | R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 | mammalian expression of SARS-CoV-2 soluble spike trimer protein |
| 166011 | R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G | mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant) |
| 166012 | R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2 | mammalian expression of SARS-CoV soluble spike trimer protein |
| 166013 | R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2 | mammalian expression of MERS-CoV soluble spike trimer protein |
| 166014 | R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2 | mammalian expression of HCoV-HKU1 soluble spike trimer protein |
| 166015 | R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2 | mammalian expression of HCoV-OC43 soluble spike trimer protein |
| 166016 | R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2 | mammalian expression of HCoV-229E soluble spike trimer protein |
| 166017 | R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2 | mammalian expression of HCoV-NL63 soluble spike trimer protein |
| 170188 | R620-M01-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.1.7 | Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant) |
| 170195 | R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 | Mammalian expression of SARS-CoV-2 soluble spike trimer protein |
| 170196 | R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351 | Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant) |
| 170197 | R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7 | Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant) |
| 170198 | R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351 | Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant) |
| 170200 | R620-M21-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.429 | Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.429 variant) |
| 170201 | R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G | Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant) |