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Transcriptional readout of neuronal activity via an engineered Ca(2+)-activated protease.

Sanchez MI, Nguyen QA, Wang W, Soltesz I, Ting AY
Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 10.1073/pnas.2006521117. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158699scFLARE1-p2A-eGFPMammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
158700scFLARE2Neuronal expression of scFLARE2 component
158701TRE-mCherry-p2A-bReaches-TSReporter construct
158702TRE-mCherry-p2A-Chrimson-TSReporter construct
158703TRE-mCherry-p2A-eNpHR3.0-TSReporter construct
158738scFLARE1Mammalian expression of scFLARE1 component

Antibodies from Article