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NTR 2.0: a rationally engineered prodrug-converting enzyme with substantially enhanced efficacy for targeted cell ablation.

Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, Ji H, Ackerley DF, Mumm JS
Nat Methods. 2022 Feb;19(2):205-215. doi: 10.1038/s41592-021-01364-4. Epub 2022 Feb 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
1586515xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2making transgenics expressing a UAS driven YFP and NTR 2.0
1586525xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2making transgenics expressing a UAS driven eGFP and NTR 2.0
1586535xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2making transgenics expressing a UAS driven mCherry and NTR 2.0
1586545xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFPmaking transgenics expressing a QUAS driven YFP and NTR 2.0

Antibodies from Article