Dynamic control over feedback regulatory mechanisms improves NADPH flux and xylitol biosynthesis in engineered E. coli.
Li S, Ye Z, Moreb EA, Hennigan JN, Castellanos DB, Yang T, Lynch MD
Metab Eng. 2021 Jan 15. pii: S1096-7176(21)00013-6. doi: 10.1016/j.ymben.2021.01.005.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158609 | pCDF-pntAB | Low phosphate induction of pntAB |
| 158611 | pCASCADE-xylA | Low phosphate inducible gRNA silencing the xylA promoter |
| 158612 | pCASCADE-udhA-xylA | Low phosphate inducible gRNA array to silence the xylA and udhA promoters |
| 158613 | pCASCADE-gltA2-xylA | Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters |
| 158614 | pCASCADE-zwf-xylA | Low phosphate inducible gRNA array to silence the xylA and zwf promoters |