CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants.
Pan C, Wu X, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang Y, Cheng Y, Shih PM, Qi Y
Nat Plants. 2021 Jul;7(7):942-953. doi: 10.1038/s41477-021-00953-7. Epub 2021 Jun 24.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158393 | pYPQ131-tRNA2.0 | Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
| 158394 | pYPQ132-tRNA2.0 | Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
| 158395 | pYPQ133-tRNA2.0 | Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
| 158396 | pYPQ134-tRNA2.0 | Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
| 158397 | pYPQ135-tRNA2.0 | Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
| 158398 | pYPQ136-tRNA2.0 | Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
| 158400 | pYPQ143-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAs |
| 158402 | pYPQ144-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs |
| 158403 | pYPQ145-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs |
| 158404 | pYPQ146-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs |
| 158406 | pYPQ141-ZmUbi-RZ-Aac.4 | Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter |
| 158407 | pYPQ141-ZmUbi-RZ-Aa3.8.5 | Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter |
| 158408 | pYPQ-dpcoCas9-Act3.0 | CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
| 158409 | pYPQ-dpcoCas9-TV | CRISPR-dpcoCas9 fused with TV activator for transcriptional activation |
| 158410 | pYPQ-dpcoCas9-SunTag | CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation |
| 158411 | pYPQ-dpcoCas9-EV2.1 | CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation |
| 158413 | pYPQ-dAaCas12b-Act3.0 | CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
| 158414 | pYPQ-dzCas9-Act3.0 | CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
| 158415 | pYPQ-dzCas9-NG-Act3.0 | CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
| 158416 | pYPQ-dSpRY-Act3.0 | CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
| 158578 | pYPQ142-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs |
| 167158 | pYPQ134B2.0 | Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
| 167159 | pYPQ135B2.0 | Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
| 167160 | pYPQ136B2.0 | Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
| 167161 | pYPQ141D-gRNA2.1 | Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter |