Real-time monitoring of peroxiredoxin oligomerization dynamics in living cells.
Pastor-Flores D, Talwar D, Pedre B, Dick TP
Proc Natl Acad Sci U S A. 2020 Jun 29. pii: 1915275117. doi: 10.1073/pnas.1915275117.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158224 | pcDNA3.1(−) mCER-Prx2 | Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells) |
| 158225 | pLPCX mCER-Prx2 | Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation) |
| 158226 | pET-SUMO mCER-Prx2 | Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression) |
| 158227 | pLPCX mCER-Prx1 | Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation) |
| 158228 | pLPCX roGFP2-Prx2(C172A) | Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation) |
| 169230 | pLPCX roGFP2-Prx2 | Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2 for cytosolic expression in mammalian cells (for stable cell line generation) |