SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition.
Cieri D, Vicario M, Giacomello M, Vallese F, Filadi R, Wagner T, Pozzan T, Pizzo P, Scorrano L, Brini M, Cali T
Cell Death Differ. 2018 Jun;25(6):1131-1145. doi: 10.1038/s41418-017-0033-z. Epub 2017 Dec 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164119 | OMMGFP 1-10 | Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM |
| 164120 | SacI ML GFP Strand 11 Long | Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10 |
| 164121 | SacI ML GFP Strand 11 Short | Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10 |