CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria.

Wang G, Zhao Z, Ke J, Engel Y, Shi YM, Robinson D, Bingol K, Zhang Z, Bowen B, Louie K, Wang B, Evans R, Miyamoto Y, Cheng K, Kosina S, De Raad M, Silva L, Luhrs A, Lubbe A, Hoyt DW, Francavilla C, Otani H, Deutsch S, Washton NM, Rubin EM, Mouncey NJ, Visel A, Northen T, Cheng JF, Bode HB, Yoshikuni Y
Nat Microbiol. 2019 Dec;4(12):2498-2510. doi: 10.1038/s41564-019-0573-8. Epub 2019 Oct 14. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
158207	pW17	2lox landing pad plasmid with mariner transposon and transposase used for CRAGE
158210	pW34	LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.
158211	pW5Y_Apr	BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites