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A rationally enhanced red fluorescent protein expands the utility of FRET biosensors.

Mo GCH, Posner C, Rodriguez EA, Sun T, Zhang J
Nat Commun. 2020 Apr 15;11(1):1848. doi: 10.1038/s41467-020-15687-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
173014pcDNA3-StagRFPRationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.
173015pcDNA3-GR-AKAR3Improved green-red FRET-based PKA activity reporter.
173016pcDNA3-Lyn-GR-AKAR3Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.
173017pcDNA3-GR-ICUE2Improved green-red FRET based cAMP indicator.
173018pcDNA3-Tsapphire-StagRFP-SrcARLong-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.
173019pcDNA3-smURFP-StagRFP-EKAR-EVRed/far-red FRET-based ERK activity reporter for multiplexed imaging applications.
173020pcDNA3-Venus-StagRFP-AktARYellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

Antibodies from Article