Reporter system architecture affects measurements of noncanonical amino acid incorporation efficiency and fidelity.
Potts KA, Stieglitz JT, Lei M, Van Deventer JA
Mol Syst Des Eng. 2020 Feb 1;5(2):573-588. doi: 10.1039/c9me00107g. Epub 2020 Jan 23.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158126 | pCTCON2-BYG | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct) |
| 158127 | pCTCON2-BXG | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct) |
| 158130 | pCTCON2-GFP | Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct) |
| 158131 | pCTCON2-GFP-TAG | Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct) |
| 158132 | pCTCON2-BXG-altTAG | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct) |
| 158134 | pCTCON2-BXG-altTAG2 | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct) |
| 158136 | pCTCON2-BXG-altTAG3 | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct) |
| 158137 | pCTCON2-BXG-altTAG4 | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct) |
| 158138 | pCTCON2-BXG-2TAG | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct) |
| 158141 | pCTCON2-Aga1p | Expresses Aga1p in yeast |
| 158142 | pCTCON2-Aga1p-FAPB2.3.6 | Yeast display reporter for non-display yeast strains (wild-type construct) |
| 158143 | pCTCON2-Aga1p-FAPB2.3.6L1TAG | Yeast display reporter for non-display yeast strains (TAG construct) |
| 158144 | pRS416-BYG | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct) |
| 158145 | pRS416-BXG | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct) |
| 158146 | pRS416-BXG-altTAG | Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct) |
| 158147 | pRS416-Aga1p-FAPB2.3.6 | Yeast display reporter for non-display yeast strains (wild-type construct) |
| 158148 | pRS416-Aga1p-FAPB2.3.6L1TAG | Yeast display reporter for non-display yeast strains (TAG construct) |
| 160926 | pCTCON2-GXB | Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct) |
| 160927 | pCTCON2-GYB | Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct) |