Targeted Diversification in the S. cerevisiae Genome with CRISPR-Guided DNA Polymerase I.
Tou CJ, Schaffer DV, Dueber JE
ACS Synth Biol. 2020 Jun 16. doi: 10.1021/acssynbio.0c00149.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158073 | pyEvolvR (enCas9-PolI5M) | Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning. |