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Donor plasmids for phenotypically neutral chromosomal gene insertions in Enterobacteriaceae.

Holden ER, Wickham GJ, Webber MA, Thomson NM, Trampari E
Microbiology (Reading). 2020 Nov 23. doi: 10.1099/mic.0.000994. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158058pDOC-K-glmSDonor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker
158059pDOC-K-glmS-GFPforDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker
158060pDOC-K-glmS-GFPrevDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker
158061pDOC-K-glmS-lacIZDonor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome
158062pDOC-K-glmS-mPlumDonor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

Antibodies from Article