Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen.
Song Q, Ni K, Liu M, Li Y, Wang L, Wang Y, Liu Y, Yu Z, Qi Y, Lu Z, Ma L
Genome Biol. 2020 Jun 8;21(1):136. doi: 10.1186/s13059-020-02044-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157981 | Direct-seq_lentiGuide-Puro-Tail-8A8G | Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone. |
| 157982 | Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G | Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone. |
| 157983 | Direct-seq_lentiGuide-Puro-Loop2-8A8G | Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone. |
| 157984 | Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G | Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone. |
| 157985 | Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G | Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone. |
| 157986 | Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G | Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone. |