A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets.
Zhou P, Chan BKC, Wan YK, Yuen CTL, Choi GCG, Li X, Tong CSW, Zhong SSW, Sun J, Bao Y, Mak SYL, Chow MZY, Khaw JV, Leung SY, Zheng Z, Cheung LWT, Tan K, Wong KH, Chan HYE, Wong ASL
Cell Rep. 2020 Aug 11;32(6):108020. doi: 10.1016/j.celrep.2020.108020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157975 | pAWp92 | pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI} |
| 157976 | pAWp28-v1 scaffold | pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones. |
| 157977 | pAWp100 | pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI} |
| 157978 | pAWp102-v2 scaffold | pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI} |
| 157980 | pAWp40 | pFUGW-EFSp-BFP-{SbfI} |