One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening.
Francino-Urdaniz IM, Steiner PJ, Kirby MB, Zhao F, Haas CM, Barman S, Rhodes ER, Leonard AC, Peng L, Sprenger KG, Jardine JG, Whitehead TA
Cell Rep. 2021 Aug 31;36(9):109627. doi: 10.1016/j.celrep.2021.109627. Epub 2021 Aug 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157971 | SARS-CoV-2 Spike (S) Ectodomain Library A | Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation |
| 157972 | SARS-CoV-2 Spike (S) Ectodomain Library B | Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation |
| 157973 | SARS-CoV-2 Spike (S) Ectodomain Library C | Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation |
| 168776 | SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 1 | Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations |
| 168777 | SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 2 | Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations |
| 168778 | pJS697 | Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination |
| 168779 | pJS699 | Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination |
| 174293 | SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 1 (positions 333-436) | This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid. |
| 174294 | SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 2 (positions 437-527) | This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid. |
| 174295 | SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 1 (positions 333-436) | This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid. |
| 174296 | SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 2 (positions 437-527) | This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid. |
| 174298 | pIFU001 | Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination |
| 174299 | pIFU002 | Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination |