A microscopy-based kinetic analysis of yeast vacuolar protein sorting.
Casler JC, Glick BS
Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
140149 | YIplac211-Vph1-HaloTag | Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag |
140150 | YIplac211-GGA2-HaloTagx2 | Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2 |
140151 | YIplac211-Sec7-mScarlet2Ix6 | Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6 |
140152 | YIplac211-Mup1-mScarlet2I | Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I |
140153 | YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL | Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus |
140154 | YIplac204-pOst1-ESCargo(Crimson) | Expresses a vacuolar cargo for yeast at the TRP1 locus |