Protein-RNA networks revealed through covalent RNA marks.
Lapointe CP, Wilinski D, Saunders HA, Wickens M
Nat Methods. 2015 Dec;12(12):1163-70. doi: 10.1038/nmeth.3651. Epub 2015 Nov 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220434 | RT-1 | Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3) |
| 220438 | RT-2 | Base construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3) |
| 220440 | RT-3 | Base construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3) |
| 220442 | RT-5 | Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank) |
| 220443 | RT-4 | Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene. |