Aurora kinase A localises to mitochondria to control organelle dynamics and energy production.

Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier M
eLife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
157749	pcDNA 3.1 AURKA deltaNter	Expression of AURKA without Nter in mammalian cells
157750	pcDNA 3.1 mitoAURKA	Expression of AURKA targeted to mitochondria
157751	pcDNA 3.1 AURKA Lys162Met	Expression of kinase-dead AURKA K62M in mammalian cells
157752	pcDNA 3.1 mitoAURKA Lys162Met	Expression of kinase-dead AURKA K62M in mitochondria
157753	pEGFP N1 AURKA deltaNter	Expression of AURKA without Nter, fused to GFP in mammalian cells
157754	pEGFP N1 mitoAURKA	Expression of AURKA:GFP targeted to mitochondria
157755	pEGFP N1 AURKA 1-110	Expression of AURKA Nter (aa1-110):GFP in mammalian cells
157756	pEGFP N1 AURKA 1-30	Expression of AURKA Nter (aa1-30):GFP in mammalian cells
157757	pCMV7 mitoDendra2	Expression of the fluorescent protein Dendra2 targeted to mitochondria
157758	pmCherry C1 Omp25	Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells
157759	pmCherry N1 MFN2	Expression of MFN2:mcherry in cells
157760	pmCherry C1 MFF	Expression of mcherry:MFF in cells
157761	pmCherry N1 TOMM20	Expression of TOMM20:mcherry in cells
157762	pmCherry N1 TOMM22	Expression of TOMM22:mcherry in cells
157767	pCMV AURKA-mTurq2	Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter
157768	pCMV ShadowG-AURKA-mTurq2	Expression of AuroraA kinase biosensor under CMV promoter
157769	pCMV ShadowG-AURKA K162M-mTurq2	Expression of AuroraA kinase-dead biosensor under CMV promoter
157770	pCMV ShadowY-AURKA-mTurq2	Expression of AuroraA kinase biosensor under CMV promoter
157771	pCMV ShadowY-AURKA K162M-mTurq2	Expression of AuroraA kinase-dead biosensor under CMV promoter
157772	pCMV superYFP-AURKA-mTurq2	Expression of AuroraA kinase biosensor under CMV promoter
157773	pCMV superYFP-AURKA K162M-mTurq2	Expression of AuroraA kinase-dead biosensor under CMV promoter
157774	pCMV mTurquoise2-shadowG tandem	Produces a fusion between mTurquoise2 and shadowG. It can be used as a positive control for FRET (using FLIM)
157775	pCMV mTurquoise2-shadowY tandem	Produces a fusion between mTurquoise2 and shadowY. It can be used as a positive control for FRET (using FLIM)
157776	pCMV mTurquoise2-superYFP tandem	Produces a fusion between mTurquoise2 and superYFP. It can be used as a positive control for FRET (using FLIM)