Debra Hansen Lab: Empty vectors with BseRI sites avoids cloning artifacts in final protein sequence
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157739 | pMBPcyto-TEV-BseRI | empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites |
| 157740 | pT7-pelB | empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites |
| 157741 | pelBHisTEV | empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites |
| 159428 | pFastBac1-melHis10TEV | empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites |