Debra Hansen Lab: Empty vectors with BseRI sites avoids cloning artifacts in final protein sequence

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
157739	pMBPcyto-TEV-BseRI	empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
157740	pT7-pelB	empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites
157741	pelBHisTEV	empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
159428	pFastBac1-melHis10TEV	empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites