Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex.
Yang B, Liu H, Liu Z, Doenen R, Nash MA
Nano Lett. 2020 Nov 16. doi: 10.1021/acs.nanolett.0c04178.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157672 | pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR | Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag. |
| 157673 | pQE80L-Coh-His-SpyTag | Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag. |
| 157674 | pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher | Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag. |
| 157675 | pQE80L-His-SpyTag-XMod-Doc(WT) | Expression of XMod-Doc wild type with His6x tag and SpyTag. |
| 157676 | pQE80L-His-SpyTag-XMod(XL2V)-Doc | Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag. |