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Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter.

Eastman AE, Chen X, Hu X, Hartman AA, Pearlman Morales AM, Yang C, Lu J, Kueh HY, Guo S
Cell Rep. 2020 Jun 23;31(12):107804. doi: 10.1016/j.celrep.2020.107804. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
157669pSCMV-H2B-FT-FastRetroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
157670pSCMV-H2B-FT-MediumRetroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
157671pSCMV-H2B-FT-SlowRetroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Antibodies from Article