Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation.

Brion C, Lutz SM, Albert FW
Elife. 2020 Nov 16;9. pii: 60645. doi: 10.7554/eLife.60645. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
157656	Plas-gRNA-LEU2	GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence
157658	Plas-CRISPR_reporter	CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene
157659	Plas-gRNA-Z3EV	GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.