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Regulation of mRNA export through API5 and nuclear FGF2 interaction.

Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI
Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
157655pET28b-API5Expresses API5 in E.coli cell
157657pHis-LMW_FGF2Expresses FGF2 in E.coli cell
157660pGEX-API5Expresses API5 in E.coli cell
157661pGEX-UAP56Expresses UAP56 in E.coli cell
157662pCAG-F-API5Expresses API5 in mammalian cell
157663pCAG-HA-LMW-FGF2Expresses FGF2 in mammalian cell
157664API5-CRISPRExpresses Cas9 and sgRNA targeting API5
157665shAPI5#1Tet inducible knockdown of API5
157666shAPI5#2Tet inducible knockdown of API5
157667shFGF2#1Tet inducible knockdown of FGF2
157668shFGF2#2Tet inducible knockdown of FGF2

Antibodies from Article