Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering
Priode JH, Haynes KA
Protocols.io, Jan 19, 2021
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157649 | GGDest1-Amp | Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert |
| 157650 | GGDest2-Amp | Golden Gate assembly of a pET28-compatible fusion-protein ORF insert |
| 157651 | GGDest3-Amp | Golden Gate assembly of a pTXB1 compatible fusion-protein ORF insert |
| 157652 | GGDestX1-Amp | Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins |
| 157654 | GGDestX2-Amp | Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA |